Pgps3 | Unique Restriction Sites [hot]

| Enzyme | Problem | |--------|---------| | | Cuts twice (once in MCS, once in the pSa origin of replication). | | EcoRV | Cuts three times (unexpectedly in the LacZ alpha fragment and the kanamycin resistance gene). | | ClaI | Cuts twice (in the MCS and the ColE1 origin). |

Happy cloning! Have you found a rare unique site in PGPS3 that I missed? Or a tricky methylation issue? Drop a comment below or tag me on Twitter @PlantBiotechLab – let’s build a better restriction map together. pgps3 unique restriction sites

Just remember: no SalI, no EcoRV, and always verify your map. When in doubt, a quick virtual digest takes 30 seconds and saves three days of failed ligations. | Enzyme | Problem | |--------|---------| | |